NLRP1在胎膜早破患者胎膜及胎盘组织中的表达及其临床意义

摘要

Objective To investigate the levels of NOD -like receptor protein -1(NLRP1)in the fetal membrane and placenta of patients with premature rupture of membranes(PROM), and to find out the role of NLRP1 in PROM. Methods A total of 60 PROM patients who delivered in our hospital from October 2015 to September 2016 selected,in-cluding 30 patients with preterm premature rupture of the membrane(PPROM)(gestational age:28 to 36 weeks)and 30 patients with term premature rupture of membranes(TPROM)(more than 37 weeks).Meanwhile, another 60 pa-tients without PROM were selected and divided into two groups(n=30):a preterm control group(28 to 36 weeks)and a term control group(more than 37 weeks).The levels of NLRP1 in the placenta and fatal membrane were measured by immunohistochemistry -streptavidin perosidase method.The amount of NLRP1 mRNAin the placenta and fatal membrane were examined by RT-PCR.Results NLRP1 mainly existed in the epithelial cells and mesenchymal cells with little differentiation of the fatal membrane.The TPROM group showed a positive rate of NLRP1 which was higher than those in other groups(P<0.05).The PPROM group and the preterm control group were statistical different(P<0.05).No sta-tistical difference was found between the PPROM group and the term control group(P <0.05).The TPROM group showed a remarkably higher level of NLRP 1 mRNA than other groups(P<0.05).The level of NLRP1 mRNA was high-er in the PPROM group than that in the preterm control group(P<0.05).No statistical difference was found between the PPROM group and the term control group(P<0.05).Conclusion The increase of NLRP1 expression in the pla-centa and fetal membrane may be related to PROM.The mechanisms of PPROM and TPROM might be different.%目的 通过研究胎膜早破患者胎膜及胎盘组织中NOD样受体蛋白1(NOD-like receptor protein -1, NLRP1)的表达情况,探讨其在胎膜早破发病中可能发挥的作用.方法 选取2015年10月—2016年9月住院分娩的胎膜早破患者共60例,其中未足月胎膜早破患者30例(28周≤发病孕周<37周),足月胎膜早破患者30例(发病孕周≥37周).另随机选择同时期无胎膜早破孕妇共60例,根据孕周不同分为未足月对照组(28周≤孕周<37周)30例和足月对照组(孕周≥37周)30例.利用免疫组化链霉菌抗生物素蛋白-过氧化物酶连接(SP)法检测胎膜及胎盘组织中NLRP1的表达情况;利用反转录-聚合酶链式反应(RT-PCR)检测胎膜及胎盘组织中NLRP1 mRNA的表达水平.结果 NLRP1主要存在于胎膜上皮细胞和分化程度很低的间充质细胞及胎盘的滋养细胞和合体滋养细胞.足月胎膜早破组胎膜和胎盘组织中NLRP1表达强度显著高于其他3组(P<0.05);未足月胎膜早破组与未足月对照组的差异有统计学意义(P<0.05);未足月对照组与足月对照组的差异无统计学意义(P>0.05).足月胎膜早破组胎膜及胎盘组织中NLRP1 mRNA的表达水平显著高于其他3组(P<0.05);未足月胎膜早破组显著高于未足月对照组(P<0.05);未足月对照组与足月对照组的差异无统计学意义(P>0.05).结论 NLRP1在胎盘和胎膜中的表达升高可能与胎膜早破的发生有关;足月和未足月胎膜早破的发生机制可能不同.