肿瘤坏死因子受体基因多态性与汉族人银屑病易感性相关研究

摘要

目的 研究肿瘤坏死因子受体(TNFR)基因多态性与汉族人银屑病易感性的关系.方法 采用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)的方法,检测107例河南豫北地区汉族银屑病患者(病例组)和110名健康体检者(对照组)TNFR基因2个单核苷酸多态性(SNP)基因座的等位基冈及基因型频率,用SPSS 13.0软件分析2个基因座多态性与银屑病遗传易感性的关系.结果 TNFR2 +676基因座基因型频率和等位基因频率分布在病例组和对照组间的差别有统计学意义(P＜0.001)；TNFR1 -329基因座基因型以及等位基因频率与对照组比较差别无统计学意义(P＞0.05).结论 河南豫北地区汉族人群中TNFR2+676基因座多态性与汉族人银屑病易感性相关.%Objective To investigate the association of tumor necrosis factor receptor (TNFR) gene polymorphisms with the genetic susceptibility of psoriasis vulgar(PV)in Han population. Methods The allele gene frequency and genotypic frequency at two single nucleotide polymorphism (SNP) situs of TNFR in 107 PV patients(case group) and 110 heathy person (control group) of Han people in northern of Henan province were detected by polymerase chain reaction (PCR) and restriction fragment length polymorphisms (RFLP) techniques, and the association of the polymorphisms at two SNP situs and the susceptibility to PV were analysed with SPSS 13.0 soft. Results The distribution of genotypes and allele of the SNP at locus + 676 of TNFR2 in case group was significantly different from that in control group (P <0. 001). There was no significant difference in the distribution of genotype for the TNFR1 -329 locus between the case group and control group,and examination of the allele frequencies also failed to show a difference between two groups (P > 0. 05 ). Conclusion These results indicate that the SNP at locus +676 of TNFR2 is associated with PV in Han people of northern Henan province.