目的 探讨黄芪对脑缺血再灌注损伤大鼠神经细胞凋亡和凋亡基因c-fos、Bcl-2表达的影响.方法 40只Wistar大鼠,体质量180-220 g,随机分为黄芪组、缺血4 h再灌注1 h组(Ⅰ组)、缺血4 h再灌注12 h组(Ⅱ组)、缺血4 h再灌注24 h组(Ⅲ组)和对照组.线栓法建立脑缺血再灌注损伤大鼠模型.切片分别行HE染色,应用免疫组织化学染色检测神经细胞凋亡基因c-fos、Bcl-2蛋白表达.结果 对照组、Ⅰ组脑细胞未见明显变化,Ⅱ组轻度脑细胞水肿,Ⅲ组脑细胞明显水肿,黄芪组损伤区范围变小,肿胀减轻.与对照组比较,其余各组脑细胞c-fos和Bcl-2阳性表达率增高(P＜0.01)；黄芪组c-fos和Bcl-2阳性表达率较Ⅱ、Ⅲ组降低(P＜0.01).Ⅰ、Ⅱ、Ⅲ组和黄芪组细胞凋亡指数较对照组明显增加(P＜0.01)；黄芪组较Ⅱ、Ⅲ组明显降低(P＜0.01).结论 黄芪在脑缺血再灌注损伤后可抑制c-fos表达、增加Bcl-2表达,减轻脑细胞凋亡.%Objective To explore the effects of astragalus on apoptosis and c-fos, Bcl-2 expression after focal cerebral ischemia-reperfusion injury in rats. Methods Forty male Wistar rats( 180 - 220 g) were randomly divided into astragalus group ,4 hours ischemia/1 hour reperfusion group (group I) ,4 hours ischemia/12 hours reperfusion group ( group Ⅱ ) ,4 hours ischemia/24 hours reperfusion group (group Ⅲ) and control group. The model of cerebral ischemia-reperfusion in rats were made by a suture-occluded method. The microtome section were given hematoxylin eosin( HE) staining. The expressions of c-fos and Bcl-2 were detected by immunohistochemistry staining. Results HE staining showed that the control group and group I was no significant change in brain cells, and mild edema of brain cells appeared in group Ⅱ , obviously edema appeared in group Ⅲ. The damage area of astragalus group was smaller and edema reduced. Compared with the control group, the positiveexpression rates of c-fos and Bcl-2 of other four groups were increased (P <0. 01) ;the positive expression rates of c-fos and Bcl-2 of astragalus group were lower than those in group Ⅱ and group Ⅲ (P <0.01). The apoptotic index of group Ⅰ, group Ⅱ , group Ⅲ and astragalus group was significantly higher than that of the control group (P < 0.01) ;the apoptotic index of astragalus group was significantly lower than that of group Ⅱ and group Ⅲ (P < 0.01). Conclusion Astragalus can inhibit the expression of c-fos and increase the expression of Bcl-2 in cerebral ischemia reperfusion and lessen brain cell apoptosis.
展开▼
