原发性肝癌患者髓过氧化物酶活性和肿瘤坏死因子-α水平变化及临床意义

摘要

Objective To investigate the changes and clinical significance of myeloperoxidase ( MPO ) activity and tumor necrosis factor-α(TNF-α) level in patients with primary hepatic cancer( PHC). Methods Fifty-one samples of cancerous tissues and normal benign hepatic tissues (more than 5 cm from the edge of the tumor) were selected respectively. The activity of MPO was detected by chromatometry and the level of TNF-α was detected by immunohistochemical method. Results The MPO activity and TNF-α level in hepatic cancer tissues were significantly lower than those in adjacent normal hepatic tissues (P ＜0.01) ,the MPO activity in well and moderately differentiated hepatic cancer tissues was significantly higher than that in poorly differentiated hepatic cancer tissues(P ＜0.01) ,and the MPO activity in hepatic cancer tissues of patients with distant metastasis was significantly lower than that in hepatic cancer tissues of patients without distant metastasis (P ＜ 0. 05). The positive expressions of TNF-α in well and moderately differentiated hepatic cancer tissues were significantly higher than that in poorly differentiated hepatic cancer tissues(F ＜0. 01 ) ,and the positive expression of TNF-α in hepatic cancer tissues of patients with distant metastasis was significantly lower than that in hepatic cancer tissues of patients without distant metastasis (P ＜ 0.01). Conclusion MPO activity and TNF-α level can be used as the indicators for evaluating the development, differentiation and metastasis of PHC.%目的 探讨原发性肝癌(PHC)患者髓过氧化物酶(MPO)活性和肿瘤坏死因子-α(TNF-α)水平的变化及临床意义.方法 选择51例PHC患者手术切除的癌变组织及距离肿瘤边缘5 cm以上的正常良性肝组织,采用比色法检测组织中MPO活性,免疫组织化学法检测TNF-α的表达.结果 肝癌组织中MPO活性和TNF-α的表达显著低于癌旁良性肝组织(P＜0.01)；高、中分化肝癌组织中MPO活性显著高于低分化肝癌组织(P＜0.01),有远处转移的肝癌组织中MPO活性显著低于无远处转移肝癌组织(P＜0.05).TNF-α在高、中分化肝癌组织中的阳性表达显著高于低分化肝癌组织(P＜0.01),有远处转移的肝癌组织中TNF-α阳性表达水平显著低于无远处转移的肝癌组织(P＜0.01).结论 MPO活性和TNF-α的表达可以作为判断PHC发生发展、分化程度及转移的检测指标.