ANTITUMOR MECHANISM OF GEM10 BY THE NATURAL KILLER ACTIVITY AND INTERLEUKIN-2 PRODUCTION

摘要

Objective To investigate the anti-tumor effects of GeM10 by the natural killer(NK) cells activities and the production of Interleukin-2 (IL-2) in peripheral blood mononuclear cells (PBMNCs). Methods Assay of human NK cells activities by dye rejection assay in vitro and production of IL-2 in PBMNC by IL-2 bioassay with IL-2 dependent cell line CTLL2 and MTT colorometric method. Results GeM10 could significantly stimulate NK activities (60μg*mL -1 GeM10: 17.077±7.665, 120μg*mL-1 GeM10: 24.9±13.04; control: 7.72±4.64, P<0.05). GeM10 could up-regulate the production of IL-2 of PBMNCs in tumor patients(60μg*mL-1 GeM10: 2.965±1.183; 120μg*mL-1 GeM10: 2.28±0.847; control: 1.792±0.823, P<0.05).Conclusion The GeM10 not only can stimulate the NK activities but also increase the IL-2 production by PBMNCs in tumor patients. These findings indicate that the GeM10 may have promise as an anti-tumor drug and a biological response modifier in clinic.