Pre-concentration and determination of amitriptyline residues in waste water by ionic liquid based immersed droplet microextraction and HPLC

摘要

This paper describes a new approach for the determination of amitriptyline in wastewater by ionic liquid based immersed droplet microextraction(IL-IDME) prior to highperformance liquid chromatography with ultraviolet detection.1-Hexyl-3-methylimidazolium hexafluorophosphate([C6MIM][PF6]) was used as an ionic liquid.Various factors that affect extraction,such as volume of ionic liquid,stirring rate,extraction time,pH of the aqueous solution and salting effect,were optimized.The optimal conditions were as follows:microextraction time,10 min;stirring rate,720 rpm;pH,11;ionic drop volume,100 mL;and no sodium chloride addition.In quantitative experiments the method showed linearity in a range from 0.01 to 10 μg/mL,a limit of detection of 0.004 μg/mL and an excellent pre-concentration factor(PF) of 1100.Finally,the method was successfully applied to the determination of amitriptyline in the hospital wastewater samples.