Characteristics of Hyaluronic Acid Derivative Cross-linked by Polyethylene Glycol

摘要

Characteristic and dynamic viscosities of Hyaluronic acid (HA) derivative modified by polyethylene glycol (PEG) were tested with different reaction times (6 h,12 h,18 h and 24 h), different molar ratio of HA/PEG (1/10,1/5,1/3 and 1/2), different molecular weight of PEG(400,6 000 and 20 000) and mass fraction is 0.4% by Wushi Viscosimeter and L-90 Rheometer at 25 ℃. Characteristic viscosity of HA derivative had the largest value in 12 h, which decreased with increasing of PEG molecular weight, but its aqueous dynamic viscosity increased with increment of PEG molecular weight. Meanwhile, we tested dynamic mechanic properties of HA derivative by 3ARES3 Rheometer at 25 ℃ to study viscoelastic changes and to compare change difference from viscosity to elasticity with the changes of vibration frequency between unmodified HA and HA derivative. Change from low vibrated frequency to high one of solution resulted in change from viscosity to elasticity of solution. In conclusion, as to the rheological properties, structure-modified HA derivative meets the requirement of biomaterial.