Fabrication, in vitro Degradation and Cytotoxic Assay of Different Cystalline Phases Calcium Polyphosphate

摘要

Fabrication, in vitro degradation and cytotoxic assay of different crystalline phases calcium polyphosphate （CPP） were reported. The CPP ceramics were fabricated by crystallizing the amorphous frits, and sintered at 550 ℃,875 ℃,1000 ℃ for 1 h to obtain the γ-CPP, β-CPP and α-CPP respectively. The effects of the different crystalline phases on their weight loss and released PO 3-4 were investigated during the degradation. And the surface change was observed by the SEM. The osteoblastic ROS17/2.8 cell line was used to estimate the cytotoxicity of CPP. The effects of CPP on cells’ proliferation were evaluated by using MTT assay. The experimental results showed that γ-CPP, β-CPP and α-CPP did not exert cytotoxic effect on the cells. In addition, the proliferation of the growth of ROS17/2.8 cells on β-CPP was optimal.