Effects of Apatite Nanocrystals on Proliferation, Subcellular Structure, Protein Synthesis Activity of Human Periodontal Ligament Cells

摘要

To determine the cellular events occurring in the presence of yttrium/hydroxyapatite(Y/HA) or HA nanocrystals, human periodontal ligament (HPDL) cells were isolated and maintained in culture. The specificity of the cells was evidenced by their proliferation, subcellular structure, and deposition of extracellular matrix components. The presence of nanocrystals was significantly related to an increase in the proliferation. Moreover, the presence of Y/HA nanocrystals was significantly related to an increase in the proliferation with HA nanocrystals. Transmission electron microscopy (TEM) demonstrated the phagocytotic process of HPDL cells toward Y/HA or HA nanocrystals. The presence of Y/HA or HA nanocrystals was significantly related to an increase in the protein synthesis activity of HPDL cells.