To investigate the efficiency of clodronate modifying HA bioceramics, and to evaluate the effect of clodronate modifying HA bioceramics on the cells in vitro, clodronate modified the porous HA bioceramics for bone scaffold by chelation. The outermost layer of the specimens was analyzed by XPS and FI-IR. The depth profile was investigated by the argon-ion sputtering method. The cell culture test was conducted using MC3T3-E1 osteoblastic cells. The cells were inoculated and cultured on the scaffolds. Morphological observation of the cells, MTT test and ALP activity test evaluated the cell attachment, proliferation and activity on the materials. Characteristic peaks in XPS and FT-IR spectra indicated clodronate being immobilized on the surface of the bioceramics. The cell culture test in cell quantity and morphology indicated active proliferation of the cells on the scaffolds. The ALP activity of the cells cultured for 3d and 7d on clodronate-HA bioceramics was slightly higher than that on HA bioceramics, but the difference was not significant. This result indicated that clodronate-HA bioceramics had favorable cytocompatibility to be used as bone scaffold with potential ability to improve osteogenesis.
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