Human Soluble TRAIL Protein Inducing Apoptosis in Osteosarcoma Cell

摘要

This study is to examine the effect of human recombi-nant soluble TRAIL (TNF-related apoptosis-inducing ligand) pro-tein inducing apoptosis in MG-63 human osteosarcoma cells. The inhibitive rates of TRAIL to MG-63 cells were detected by MTT assay. The apoptosis induced by TRAIL in MG-63 human os-teosarcoma cells was analyzed with FACS and TUNEL and the apoptotic bodies were observed by transmission electron micro-scope. MTT assay showed that the inhibitive rates of 500, 1 000, 2 000 and 4 000 ng/mL TRAIL for 24 h were 10.1%, 24.3%, 50.6% and 97.7% respectively. Flow cytometric analysis showed that after MG-63 cells were treated with 2 μg/mL TRAIL for 6 h, obvious apoptotic peak would immediately appear before diploid peak. Human soluble TRAIL protein can quickly kill MG-63 os-teosarcoma cells selectively, and may have potential value for clinical treatment of osteosarcoma.