重组绿豆BBI（6-33）结构域的抗肿瘤作用分析

摘要

通过大肠杆菌外源表达绿豆BBI蛋白抗肿瘤活性结构域，研究纯化后的BBI蛋白抗肿瘤活性结构域蛋白对肿瘤细胞增殖、凋亡和迁移的影响及引起肿瘤细胞凋亡的分子机制．MTT、DAPI 染色、克隆形成、划痕实验等结果表明，重组BBI(6-33)域对A549等肿瘤细胞的增殖和迁移有抑制作用，对其凋亡则起促进作用．Western blot分析显示，重组 BBI(6-33)域可激活 caspase-3、caspase-9、p21等表达，提示其诱导肿瘤细胞凋亡作用可能主要是通过线粒体途径发挥的．%The antitumor activity domain of mung bean BBI was expressed byE.coli.In the present research,the effect of the purified mung bean BBI(6-33)domain on the proliferation,apoptosis and migration of tumor cells and the related mecha-nism were studied.MTT,DAPI staining,colony formation and scratch tests showed that the proliferitation and migrationof tumor cells treated with recombinant mung bean BBI(6-33)were significantly inhibited,while the apoptosis of tumor cells were promoted.The experiment of Western blot showed that caspase-3 and caspase-9 can be activated by recombined BBI(6-33)domain,and the expression level of p21,was increased,which suggests that recombined mung BBI(6-33)can induce tumor cells apoptosis mainly through mitochondrial pathway.