桔百颗粒（乐康宁）对雏鸡感染鸡传染性支气管炎的防治效果

摘要

【目的】评价桔百颗粒（乐康宁）对鸡传染性支气管炎（IB）的临床防治效果，为利用中药防治IB提供新思路。【方法】将130羽1日龄非免疫雏鸡随机分为桔百颗粒预防组、治疗组、药物对照组、免疫组、模型对照组和健康对照组，免疫组30羽，其他组均为20羽。除健康对照组外，其他组均于15日龄时滴鼻点眼感染鸡传染性支气管炎病毒（IBV）M41毒株，预防组在感染前5 d（10日龄）饮水给药；治疗组和药物对照组均在发病超过半数时给药治疗；免疫组则在7日龄免疫H120疫苗（免疫后一部分给药，另一部分不给药）。统计感染后各组雏鸡的发病率、保护率、治愈率及呼吸道症状评分，同时统计感染后其体重增长及免疫器官指数，并测定血清IBV特异性抗体。【结果】感染IBV后第8 d（23日龄）统计发现，预防组的保护率为50.00%，免疫不给药组的保护率为45.00%；治疗组的治愈率为66.67%，药物对照组为58.82%；免疫不给药组的呼吸道症状评分最低，预防组和治疗组次之。停药后第12 d（36日龄），治疗组雏鸡的脾脏指数显著高于其他组（P<0.05，下同）；感染IBV后第14 d（29日龄），治疗组雏鸡的IBV抗体水平达峰值，感染IBV后第21 d（36日龄）仍维持在较高水平，免疫并给药组的抗体水平显著高于免疫不给药组。【结论】桔百颗粒有效降低IB发病率及治愈患病雏鸡，是通过促进脾脏等免疫器官发育，进而促使机体抗体生成、延长抗体维持时间、增强机体免疫力来实现，尤其对于呼吸型IB具有良好的临床预防和治疗效果。%[Objective]The clinical prevention and treatment effects of Jiebai granule(trade name Lekangning) on avian infectious bronchitis(IB) were evaluated, in order to provide a new idea for using traditional Chinese medicine to prevent and cure IB. [Method]A total of 130 1-day-old non-immunized chicks were randomly divided into 6 group(Jiebai granule prevention group, treatment group, drug control group, vaccine group, model control group and healthy control group). Thirty chicks were in vaccine group and twenty chicks in other groups. Except for healthy control group, the chicks in oth-er groups were challenged oculonasally with avian infectious bronchitis virus(IBV) M41 strain at 15-day old, and Jiebai granule was added into drinking water before 5 days(at 10-day old) of challenge in prevention group. In Jiebai granule treatment group and Yinhuang oral solution treatment control group, Jiebai granule and Yinhuang oral solution were added into drinking water, respectively when more than half of the chicks were sick. In vaccine group, chicks were vaccinated with H120 vaccine at 7-day old, and partial vaccinated chicks drank water with Jiebai granule, other vaccinated chicks were treated without Jiebai granule. After IBV infection, the incidence, protection rate, cure rate and respiratory symptom scores of each group were counted. The weight gain and immune organ index, the levels of IBV antibodies in serum were analyzed. [Result]At 8 d after being infected with IBV(at 23-day old), the protection rate was 50.00% in prevention group and 45.00% in vaccine without drug group. The cure rates were 66.67%, and 58.82% in treatment group and treat-ment without drug group, respectively. The clinical symptoms score was the lowest in vaccine without drug group, followed by prevention and treatment groups. The spleen in treatment group were significantly higher than those in other groups at the 12 d after drug withdrawal(at 36-day old)(P<0.05, the same below). The antibody titers of prevention and treat-ment group were reach the peak at the 14 d post infection(dpi)(at 29-day old), and still kept at high level at 21 dpi(at 36-day old). The antibody titer of the vaccine with drug group was significantly higher than that of vaccine without drug group. [Conclusion]The Jiebai granule can reduce the incidence of IB and cure the sick chickens. At the same time, Jiebai granule can enhance immunity by promoting the development of spleen and other immune organs, promoting anti-body production and increasing the maintenance time of antibody. The Jiebai granule has a better clinical prevention and treatment effect on IB.