首页> 中文期刊>华南农业大学学报 >饲料碳、脂比例对胭脂鱼幼鱼生长及糖代谢的影响

饲料碳、脂比例对胭脂鱼幼鱼生长及糖代谢的影响

     

摘要

[Objective]This study investigated the influences of dietary carbohydrate-to-lipid m( CHO)∶m ( L) on growth performance , feed utilization and carbohydrate metabolism enzymes activities of juvenile Chinese sucker , Myxocyprinus asiaticus to detect the suitable addition proportion of m( CHO)∶m( L) to the diet of juvenile Chinese sucker .[Method]Six isonitrogenous ( crude protein 41%) and isoenergetic (16 kJ/g) diets were formulated to contain with graded levels of m( CHO)∶m( L) (0.29,0.81,1.58, 2.88 ,5.46 and 13.22 ) .Each diet was randomly fed to one treatment of Chinese sucker with an average initial body mass of (4.01 ±0.02) g for 8 weeks.Mass gain rate(MGR), specific growth rate (SGR), body composition and carbohydrate metabolism enzymes activities of juvenile Chinese sucker were detec -ted and analyzed .[Result and conclusion]The mass gain rate and specific growth rate increased initially but then decreased with the increasing dietary m( CHO)∶m( L) , thus reaching the highest in fish fed di-ets with m(CHO)∶m(L) of 5.46, which was significantly higher than those of fish fed other diets (P<0.05), whereas feed coefficient rates(FCR) showed a converse trend;the crude lipid content in whole-body of fish decreased with the increasing dietary m(CHO)∶m(L) significantly (P<0.05), while he-patic crude lipid decreased initially and then increased , reaching the lowest level in fish fed diets with m ( CHO)∶m( L) of 2.88; Hepatic triglycercide concentrations increased significantly with the increasing dietary m(CHO)∶m(L) (P<0.05), while activities of aspartate aminotransferase (AST) and alanine aminotransferase ( ALT ) increased initially and then decreased , reaching the peak with dietary m(CHO)∶m(L) of 2.88;lipid content of muscle was lowest in fish fed diet with m(CHO)∶m(L) of 13.22, which was significantly lower than in fish fed diet with m(CHO)∶m(L) of 0.29 (P<0.05).He-patic pyruvate kinase(PK) activities increased significantly with the increasing dietary m(CHO)∶m(L) ( P<0.05 ) , while phosphoenolpyruvate carboxykinase ( PEPCK ) showed a converse trend .No signifi-cant difference was observed in activities of hepatic hexokinase ( HK) and glucose-6-phosphatase ( G-6-pase ) among dietary treatments ( P>0.05 ) .Based on broken-line regression analysis of SGR against di-etary m( CHO)∶m( L) , a m( CHO)∶m( L) of 4.65 was proved to be optimal for the growth of juvenile Chinese sucker .%【目的】研究饲料中碳水化合物(CHO)与脂肪(L)比例[m(CHO)∶m(L)]对胭脂鱼Myxocyprinus asiaticus幼鱼生长、饲料利用及糖代谢酶活力的影响,确定其适宜添加比例.【方法】以m( CHO)∶m( L)为0.29、0.81、1.58、2.88、5.46和13.22的6种等氮(粗蛋白质量分数41%)等能(16 kJ/g)饲料,投喂初始体质量为(4.01±0.02) g的胭脂鱼幼鱼8周,测定并分析其对胭脂鱼幼鱼增质量率、特定生长率、鱼体营养成分和糖代谢酶活力等指标的影响.【结果和结论】随着饲料m(CHO)∶m(L)增加,胭脂鱼幼鱼的增质量率和特定生长率先上升后下降,均在m(CHO)∶m(L)为5.46时达到最高,并显著高于其他组(P﹤0.05),饲料系数呈相反趋势;全鱼粗脂肪含量随m(CHO)∶m(L)增加显著下降(P﹤0.05),肝脏粗脂肪则呈先降后升的趋势,在m(CHO)∶m(L)为2.88时达到最低;肌肉粗脂肪含量在m(CHO)∶m(L)为13.22时达最低,且显著低于0.29时(P<0.05).随m(CHO)∶m(L)增加,肝脏三酰甘油的含量显著上升(P<0.05),谷草转氨酶和谷丙转氨酶活力先升高后降低,均在m(CHO)∶m(L)为2.88时达到最高;肝脏丙酮酸激酶活力随m(CHO)∶m(L)增加显著上升(P<0.05),而磷酸烯醇式丙酮酸激酶活力则呈下降趋势,己糖激酶和葡萄糖-6-磷酸酶活力无显著变化(P>0.05).用折线回归模型拟合特定生长率和 m(CHO)∶m(L)的关系,得到胭脂鱼幼鱼获得最大生长时,饲料中m(CHO)∶m(L)为4.65.

著录项

  • 来源
    《华南农业大学学报》|2014年第3期|1-723|共8页
  • 作者单位

    华中农业大学水产学院;

    湖北武汉430070;

    华中农业大学水产学院;

    湖北武汉430070;

    中国水产科学研究院长江水产研究所;

    湖北武汉430223;

    中国水产科学研究院长江水产研究所;

    湖北武汉430223;

    中国水产科学研究院长江水产研究所;

    湖北武汉430223;

    中国水产科学研究院长江水产研究所;

    湖北武汉430223;

    中国水产科学研究院长江水产研究所;

    湖北武汉430223;

    中国水产科学研究院长江水产研究所;

    湖北武汉430223;

  • 原文格式 PDF
  • 正文语种 chi
  • 中图分类 水产动物饵料及其营养;
  • 关键词

    胭脂鱼; 碳脂比; 生长性能; 糖代谢酶;

  • 入库时间 2022-08-17 10:16:09

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