Objective]The objective of the study was to establish regeneration system of C.para-disi in vitro and to provide useful references for the application of genetic transformation tech-nique.[Method]The roots of aseptic seedlings germinated from the seeds of 10 grapefruit varie-ties were cultured in vitro to obtain regeneration plants in the medium with different hormones and concentrations.[Results]The callus were observed in the medium MS+0.5 mg/L 6-BA+0.5 mg/L NAA and Perlist had the highest callus induction frequency (87.95%).The differentia-tion of adventitious buds was observed in the medium MS+2.0 mg/L 6-BA+0.5 mg/L NAA and Star Ruby had the largest differentiation rate of adventitious buds (37.84%).The regeneration buds were multiplied in the medium MS+2.0 mg/L 6-BA+0.2 mg/L NAA.The adventitious roots were induced in the medium 1/2 MS +1.0 mg/L IBA and the regeneration plants finally were obtained.The survival rates of regenerative plant ranged from 75.47% to 88.40%.[Con-clusion]The regeneration systems of 6 grapefruit varieties were established based on the study.%【目的】建立葡萄柚（Citrus paradisi Macf．）离体再生体系，为后续遗传转化体系的建立奠定基础。【方法】以10个葡萄柚品种种子萌发的无菌苗根段为材料，在附加不同植物激素种类及浓度的培养基上进行离体培养，并获得再生植株。【结果】在 MS＋0．5 mg／L 6-BA＋0．5 mg／L NAA 培养基上培养可以诱导出愈伤组织，其中“帕利斯”的诱导率最高，达87．95％；在培养基 MS＋2．0 mg／L 6-BA＋0．5 mg／L NAA 可分化出丛生芽，其中“星路比”分化率最高为37．84％；分化出的丛生芽在 MS＋2．0 mg／L 6-BA＋0．2 mg／L NAA 培养基上可以进行增殖培养；在1／2 MS＋1．0 mg／L IBA 上可诱导出不定根，形成完整再生植株。炼苗移栽成活率均可达75．47％～88．40％。【结论】通过对10个葡萄柚品种根段离体再生培养，建立了6个品种的离体再生体系。
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