Objective: To study the feasibility of discarded embryos as a quality control in the IVF laboratory.Methods: The embryos from OPN, 1PN, and 2PN were cultured in microdrops of sequenced medium and observed daily from Day 3 to Day 6.rnResults: A total of 396 abandoned embryos were cultured. Ninety eight blastocysts were formed from Day 4 to Day 6 and the blastocyst rate was 24. 75%. The blastocyst formation rate from OPN, 1PN, 2PN, 3PN was 50. 5%(51/101), 24. 59%(30/122), 17. 07%(7/41)and 7. 58%(10/132), respectively. The zy-gote with 2PN and OPN could develope to embryos. The hatch rate was 30%(10/30)and 47. 06%(24/51), respectively. The clinical pregnancy rate of the women with blastosphere formed was 30. 98% (43/142), which was significantly higher than those without blastosphere formed(19. 71% , 28/142) (P<0. 05). The clinical pregnancy rate of the women with OPN and 2PN was 23. 24% (33/142), which was significantly higher than those without formation of blastosphere(9. 15%, 13/142)(P<0. 01).Conclusions: The discarded embryos have different development potential. They can develop into blas-tospheres. The blastosphere formation rate of OPN and 2PN can be more than 35%. It can be used as a quality control for IVF laboratory and a predictor for clinical pregnancy outcome.%目的 探讨废弃胚胎作为体外受精(IVF)实验室质控的可行性. 方法 通过囊胚序贯培养将无原核(0PN)、单原核(1PN)、3原核(3PN)和发育迟缓或停滞的2原核(2PN)的废弃胚胎进行囊胚培养,比较不同来源胚胎的囊胚形成情况. 结果 共收集396个废弃胚胎,经序贯培养形成98个囊胚(24.75％),0PN、2PN、1PN、3PN的囊胚形成率分别为50.50％(51/101)、24.59％(30/122)、17.07％(7/41)、7.58％(10/132),其中0PN、停滞的2PN的胚胎有孵出,孵出率分别为47.06 ％(24/51)和33.33％(10/30)；有囊胚形成与无囊胚形成患者的临床妊娠率分别为30.28％(43/142)、19.72％％(28/142),两者相比有统计学差异(P＜0.05)；特别是0PN+2PN患者的临床妊娠率为23.24％(33/142),显著高于无囊胚形成者的9.15％(13/142)(P＜0.01). 结论 废弃胚胎有不同程度的发育潜能,可部分发育成囊胚,特别是0PN+2PN囊胚形成率超过35％,可以作为IVF实验室质控的一种辅助方法,并可作为临床妊娠结局预测的参考指标.
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