大鼠放射性皮肤溃疡组织中p53,MDM2蛋白高表达病理研究

摘要

An animal model of radiation-induced skin ulcer was set up with 140 rats, which were locally irradiated with 35～55Gy γ-rays. The pathological changes were observed for 1 year. Imrnunohistochemical studies were performed in 72 rat radiation skin ulcer specimens using anti-p53 and anti-MDM2 protein polyclonal antibodies.The results showed that the positive rate for overexpression of p53 protein was 9.7%,and for that of MDM2 was 19.4%. The overexpression of p53 was mainly seen in the nuclei of activated squamous epithelial cells, and in fibroblasts, endotheliocytes in deeper part of the skin ulcers. The overexpression of MDM2 had the same localizations. It's suggested that the changes of p53 and MDM2, genes and proteins, may be related to the cancer transformation and poor healing of radiation-induced skin ulcers.%研究大鼠放射性皮肤溃疡组织中p53,MDM2表达.采用140只Wistar大鼠进行局部照射制备放射性皮肤溃疡动物模型,观察病变一年,然后采用p53(Goat),MDM2(兔)多克隆抗体和SP免疫组化方法检测皮肤溃疡组织中p53,MDM2的表达情况.结果表明,p53,MDM2的高表达阳性率分另为9.7%(7/72)、19.4%(14/72),两者阳性部位主要见于增生肥大的鳞状上皮细胞核、间质成纤维细胞及血管内皮细胞核.p53,MDM2基因的变化及蛋白的高表达可能与放射性皮肤溃疡癌变及不愈合有关.