从印度洋海区水深为4 517 m的深海泥样中筛选到一株细菌,菌株号为406-6.通过形态学特征和生理生化特征分析表明,菌株406-6为革兰氏阴性菌,细胞呈稍微弯曲的棒状.菌落为乳白色,圆形.菌株406-6生长需要NaCl,62 ℃时生长微弱,能利用小分子的甘氨酸作为唯一碳源生长,但不能利用L-阿拉伯糖、D-木糖、D-葡萄糖、D-半乳糖、D-甘露糖、蔗糖、D-甘露醇、D-海藻糖和D-纤维二糖作为唯一碳源.能水解酪素、明胶、脱脂奶粉、Tween 40和Tween 60,但是不能水解淀粉、Tween 20和Tween 80.16S rRNA基因序列比对分析表明,菌株406-6与Idiomarina属的I.fontislapidosi F23T相似性最高(98.0%),和其他属的相似性均低于94.0%.Neighbor-joining系统树中,菌株406-6和Idiomarina属内的菌种相聚成簇.基于以上微生物学特征分析表明,406-6为Idiomarina属内的一个潜在的细菌新物种,该研究丰富了Idiomarina属的物种多样性,为微生物资源的开发利用奠定基础.%A bacterium, designated 406-6, was isolated from sediment of the Indian Ocean at a water depth of 4517 m.Based on the results of the morphological and physiological-biochemical characterization of strain 406-6, it is Gram-negative, straight or slightly curved rods.Colonies are white and circular.Strain 406-6 did not grow without NaCl, but could slightly grow at 62°C.Strain 406-6 could utilize small molecules of glycine as sole carbon sources for growth, but not L-arabinose, D-xylose, D-glucose, D-galactose, D-mannose, sucrose, D-mannitol, D-trehalose and D-cellobiose.Hydrolyses casein, gelatin, skimmed milk, Tween 40 and Tween 60, but not starch, Tween 20 and Tween 80.Comparative analysis of the 16S rRNA gene sequence showed that strain 406-6 had the highest 16S rRNA gene sequence similarity to the type strain of Idiomarina fontislapidosi F23T (98.0%), and less than 94.0% sequence similarity to type strains of other known genus.In the neighbor-joining tree, strain 406-6 and the type strains of Idiomarina formed a coherent branch.Based on the results of the microbiological characterization of strain 406-6, it represents a potentially novel species of the genus Idiomarina.This study enriches the species diversity of the genus Idiomarina, and lays the foundation for the development and utilization of microbial resources.
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