紫杉醇脂质体与紫杉醇不同途径灌注治疗兔舌癌的疗效研究

摘要

目的:研究经兔舌动脉、耳缘静脉分别灌注紫杉醇脂质体或紫杉醇治疗兔VX-2舌癌的疗效.方法:建立兔VX-2舌癌模型,随机分为6组(n=4).其中,舌动脉灌注3组,分别灌注紫杉醇脂质体,紫杉醇,5％葡萄糖溶液;耳缘静脉灌注3组,用药同上.治疗后7d,采用体积测量、流式细胞术、免疫组织化学对舌癌病灶的治疗反应、肿瘤细胞凋亡、P53蛋白表达进行检测.数据经SPSS 20.0统计软件分析.结果:舌动脉灌注紫杉醇脂质体组有效率明显优于其他各组(P＜0.05),细胞凋亡率明显高于其它各组(P＜0.05),P53蛋白表达明显低于其它各组(P＜0.05).结论:舌动脉灌注紫杉醇脂质体进行兔VX-2舌癌治疗效果最佳.%Objective:To study the effects of the treatment for rabbit VX-2 tongue cancer with paclitaxel or paclitaxel liposome infusion through rabbit lingual artery or ear vein infusion.Methods:24 rabbits with VX-2 tongue cancer were divided into 6 groups(n =40) randomly.The rabits in 3 groups were respectively treated by intra-arterial infusion with paclitaxel,paclitaxel liposome and 5％ glucose,and those in other 3 groups by ear vein infusion with the same materials.After 7 days of treatment,the lesion volumes were measured for response evaluation,cells apotosis in the cancer tissure was detected by llowcytometry,P53 protein expression was examined by immunohistochemical staining,respectively.The data was analyzed by SPSS 20.0 software package.Results:The response rate and apotosis of the tumor cells in intra-arterial infusion with paclitaxel liposome group were higher than those in the other groups(P ＜ 0.05),P53 protein expression was lower than that of the other groups (P ＜ 0.05).Conclusion:Intra-arterial infusion through tongue artery with paclitaxel liposome is more effective than the other methods in the treatment of rabbit VX-2 tongue cancer.