Objective To obtain the optimal protocols for sperm freezing by studying different factors influencing the motility of post-thaw. Methods The effects of cryopreservative methods and concentrations of glycerol, egg yolk, and seminal plasma on the motility of post-thaw were compared. Results The rate of post-thaw motility was significantly higher in slow freezing than in fast freezing. The rate of post-thaw motility was significantly higher in the cryoprotectant containing 6％ or 7.5％ glycerol than in those of 4％ or 9％, but there was no significant difference between the two former cryoprotectants.The rate post-thaw motility did not differ significantly among the cryoprotectants containing egg yolk of 5％, 10％, and 15％, but was significantly higher than that of 0％. The rate of post-thaw motility did not differ significantly between seminal plasma of 50％ and that of 75％ ,whereas was higher than that of 0％. Conclusions Cryopreservative methods and concentrations of glycerol, egg yolk, and seminal plasma have an effect on the post-thaw motility of human sperm. The slow freeze with glycerol final concentration of 6％ ～ 7.5％, egg yolk of 5％ ～ 15％, and seminal plasma of 50％ or 75％ is feasible during the process of spermfreezing.%目的:通过对冷冻过程中影响精子冷冻复苏率的不同因素进行研究,以获取最佳的冷冻方案.方法:比较冷冻过程冷冻方法、甘油浓度、卵黄浓度及精浆浓度对精子冷冻复苏率的影响.结果:(1)缓冻法精子冷冻复苏率高于直接冷冻法,差异有显著性;(2)甘油浓度为6%或7.5%的保护剂冷冻复苏率显著高于甘油浓度为4%及9%的保护剂.但前两者间无显著差异;(3)卵黄浓度为5%,10%,15%的保护剂间冷冻复苏率无显著差异,均高于卵黄浓度为0%的保护荆,差异有显著性;(4)50%及75%精浆浓度冷冻复苏率无显著差异,均显著高于0%的精浆浓度.结论:精子冷冻过程中冷冻方法及甘油、卵黄、精浆浓度对精子冷冻复苏率均有影响.采用缓冻法,甘油终浓度6%～7.5%,、卵黄终浓度5%～15%、精浆终浓度50%或75%的精子冷冻方案是可行的.
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