目的：先前报道红细胞中存在淀粉样纤维，本文进一步探讨成熟中性粒细胞是否存在淀粉样变纤维，为解析阿尔茨海默病（AD）淀粉样变形成分子机制提供科学依据。方法：收集AD患者以及对照人群各30例，利用希森美康X5-500i血液分析仪测定血液白细胞、红细胞、血红蛋白水平以及中性粒细胞绝对值等4项指标，采集静脉EDTA-K2抗凝血分离中性粒细胞，分别测定中性粒细胞与淀粉样变特异探针之间亲和性，血浆部分用硫磺素T（ThT）检测淀粉样变荧光强度。结果：亲和试验结果显示淀粉样变荧光探针（ThT）能够与组织淀粉样变纤维特异性结合，中性粒细胞中淀粉样变纤维染色也呈现阳性反应。 AD组与健康对照组间血细胞白细胞、红细胞、血红蛋白水平以及中性粒细胞绝对值比较差异无统计学意义（P＞0．05），但是血浆淀粉样变荧光强度显著高于对照组（P ＜0．05）。结论：报道成熟中性粒细胞发现淀粉样变纤维，且AD患者血浆淀粉样变纤维水平显著增高。%Objective To investigate whether there is amyloid fibers in the mature neutrophils and to reveal the molecular mechanism of the formation of AD amyloidosis. Method Thirty cases of AD patients and 30 healthy control were enrolled. The white blood cell, the red blood cell,hemoglobin and neutrophil absolute value were determined by Sysmex X5-500i hematology analyzer. The neutrophils and amyloid variable specific probe affinity were measured, The starch like variable fluorescence intensity in plasma was dtected by the sulfur T (Thioflavin T, ThT) method. Results The affinity test results showed that the amyloidosis fluorescent probe (ThT) can be combined with tissue amyloid fibers specifically. The neutrophil amyloid fibers staining also showed a positive reaction. Compared with the AD group, no significant differences were found in white blood cell, red blood cell, hemoglobin and neutrophil absolute value level in the healthy control group The serum amyloid variable fluorescence intensity in the AD group was significantly higher than that in the control group (P < 0.05). Conclusion The amyloid fibers was found in the mature neutrophils, and the level of plasma amyloid fibers was significantly increased in the AD patients.
展开▼
