乙型肝炎肝硬化和肝癌患者肝组织HBV复制状态分析

摘要

Objective To investigate the hepatitis B virus replication status in patients with liver cirrhosis and primary liver cancer.Methods In 1982 patients with liver cancer and in 1995 with liver cirrhosis, serum HBV DNA and HBVcccDNA in liver tissues were detected.Results The serum anti-Hbe positive rates in patients with liver cirrhosis and liver cancer were 61.7% and 74%, respectively (P<0.01);in patients with serum anti-Hbe positive no matter with cirrhosis or cancer; the serum HBV DNA≤103copies/ml was 69.1%; much higher than 21.6% in patients with serum HbeAg positive (P<0.01);in patients with anti-Hbe positive , serum HBV DNA≥ 107copies / ml was 6.4%; much lower than 42.0% in HbeAg positive (P<0.01) ; in anti-Hbe positive patients , serum HBV DNA levels was 3.8 lg copies/ml, much lower than in in HbeAg positive (6.llgcopies/ml) ; in 12 patients; all were hepatic HBV DNA positive; and 8 were hepatic HBV cccDNA positive.Conclution Patients with liver cirrhosis and liver cancer have a high percentage of serum anti-Hbe positive; and HBV DNA or HBVcccDNA can be detected in liver tissues.%目的 了解肝硬化和肝癌患者HBV的复制状态.方法 在1982例肝癌和1995例乙型肝炎肝硬化患者,常规检测HBV标记物,并在其中12例患者取得肝组织进行HBVcccDNA检测.结果 肝硬化和肝癌患者抗Hbe阳性所占比例分别为61.7%和74%(P<0.01);将肝硬化和肝癌患者合并后,血清抗Hbe阳性者血清HBV DNA≤103拷贝/毫升者占69.1%,高于HBeAg阳性者的21.6%(P<0.01);抗Hbe阳性患者HBV DNA≥107拷贝/毫升者占6.4%,低于 HBeAg阳性者的42.0%(P<0.01);HBeAg阳性和抗Hbe阳性患者HBV DNA载量分别为6.1 lg拷贝/毫升和3.8lg拷贝/毫升(P<0.01);在送检的12例肝组织,HBV DNA均阳性,HBVcccDNA阳性8例.结论 肝硬化和肝癌患者抗-Hbe阳性比例较高,肝组织HBVcccDNA仍可被检出.