The pharmacokinetic study of rutin in rat plasma based on an electrochemically reduced graphene oxide modified sensor

摘要

An electrochemical method based on a directly electrochemically reduced graphene oxide(ERGO) film coated on a glassy carbon electrode(GCE) was developed for the rapid and convenient determination of rutin in plasma. ERGO was modi fi ed on the surface of GCE by one-step electro-deposition method.Electrochemical behavior of rutin on ERGO/GCE indicated that rutin underwent a surface-controlled quasi-reversible process and the electrochemical parameters such as charge transfer coefficient(α),electron transfer number(n) and electrode reaction standard rate constant(k_s) were 0.53, 2 and 3.4 s^(-1),respectively. The electrochemical sensor for rutin in plasma provided a wide linear response range of 4.70*10^(-7)-1.25*10^(-5)M with the detection limit(s/n=3) of 1.84*10^(-8)M. The assay was successfully used to the pharmacokinetic study of rutin. The pharmacokinetic parameters such as elimination rate half-life(t_(1/2)), area under curve(AUC), and plasma clearance(CL) were calculated to be 3.345±0.647 min, 5750±656.0 μg min/mL, and 5.891±0.458 mL/min/kg, respectively. The proposed method utilized a small sample volume of 10 μL and had no complicated sample pretreatment(without deproteinization), which was simple, eco-friendly, and time- and cost-efficient for rutin pharmacokinetic studies.