Objective To investigate the anticancer effects and detailed mechanisms of Saikosaponin D(SSD)in human hepatoma HepG2 cells.Methods Cell proliferation and apoptosis were tested by MTT assay and Annexin-V/PI assay respectively.The expressions of CCAAT enhancer binding protein β(C/EBPβ)and p53 were detected by RT-PCR and Western blotting.Results SSD inhibited cell proliferation in a dose-dependent manner and induced apoptosis at the concentration of 5.0 mg/L.SSD significantly increased the mRNA and protein le...
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