Objective : To investigate the effects of 1％ hydrochloric acid and 10％ nitric acid on immunohistochemical staining for several antigens. Methods: Fifteen cases of focally calcified soft tumors including three cases of thyroid papillary carcinoma, three of breast invasive ductal carcinoma, three of prostate cancer, three of colon adenocarcmcoma and three of normal lymph nodes, were selected in this study, and so were mne cases of bone and bone marrow tissue from biopsy and autopsy. Tissue samples were divided into three groups. One group was each soaked into 10％ nitric acid for 24, 42 and 48 h, another group was each incubated in 1％ hydrochloric acid for 1. 2 and 3 h. The other group which was not treated with acid was regarded as control. Then immunohistochemical staining was conducted in these samples for antigens as follows: ER, PR and Ki-67 in breast invasive ductal carcmoma; TTF-1 in thyroid papillary carcmoma; 34βE12 and P504s in prostate cancer; AE1/AE3 , P504s and Ki-67 in colon adenocarcincoma; CD3 , CD20 and Ki-67 in lymph nodes; CD3 ,CD20 , CD68 , CD235a, MPO, Ki-67 , AE1/ AE3 and TTF-1 in bone tissue. Results : After the tissues decalcificated in both acid solutions for some time, the numbers of positive cells decreased and the intensity of immunostaining became weaker, which was proportionate to time. Bone tissue was less influenced than calcified soft tissue. Different antigens showed different sensitivity to the same acid treatment. TTF-1 and Ki-67 were the two most vulnerable antigens among them; while ER, CD3 and CD20 were the least influenced. The effects of decalcification on immunostaining of one antigen were the same in different tissues. Conclusion: In most cases, both hydrochloric acid and nitric acid decalcification can lower the sensitivity of immunohistochemical staining, but have different effects on different antigens. It is better to use 10％ nitric acid for decalcification of relatively large bone tissue and to use 1％ hydrochloric acid for bone marrow tissues and bone tissues containing much more soft tissue.%目的:了解1%(体积分数)盐酸和10%(体积分数)硝酸脱钙液对多种抗原免疫组织化学染色效果的影响.方法:选择伴有灶状钙化的软组织标本包括甲状腺乳头状癌、乳腺癌、前列腺癌、结肠癌和淋巴结各3例,骨组织标本包括活检和尸检病例共9例,将样本分为3组,一组分别用10%(体积分数)硝酸处理24、42和48 h,另一组分别用1%(体积分数)盐酸处理1、2和3 h,还有一组不用酸处理作为对照组,同时进行多种抗原免疫组织化学染色,其中乳腺癌组织作ER、PR和Ki-67染色,甲状腺癌组织作TTF-1染色,前列腺癌作34βE12和P504s染色,结肠癌作AE1/AE3、P504s和Ki-67染色,淋巴结作CD3、CD20和Ki-67染色,骨组织作CD3、CD20、CD68、CD235a、MPO、Ki-67、AE1/AE3和TTF-1染色.结果:伴有钙化的软组织和骨组织内多种抗原免疫组织化学染色阳性程度均随酸处理时间的延长而有不同程度的下降,骨组织对酸处理的耐受力比软组织强;不同抗原对酸处理的敏感性不同,TTF-1和Ki-67最为敏感,ER、CD3、和CD20耐受性较好.结论:大多数情况下,盐酸和硝酸处理都会降低组织免疫组织化学染色的敏感性,但是对不同抗原的影响程度不同.大块骨组织可以采用10%(体积分数)硝酸脱钙,软组织比例较高的骨组织和骨髓活检组织尽可能使用1%(体积分数)盐酸脱钙.
展开▼
