昼夜温差对文心兰试管苗生长的影响

摘要

The plantlets of Oncidium var.‘Ning Meng Huang' in vitro were used as experimental materials to study the effects of day-night temperature differences on its growth.The results showed that the index of plantlets in vitro under the treatment that the day-night temperature difference was 3℃, including plant height, leaf length, fresh weight and dry weight of the total and shoot part, were the best than other treatment, and there was significant difference among the other treatments.The root number, fresh weight and dry weight of the root part of plantlets in vitro were better than others under the treatment that the day-night temperature difference was 12℃, and the soluble sugar and root activity of plantlets in vitro were also the biggest one.It was an increasing tendency, as the chlorophyll a, chlorophyll b, total leaf chlorophyll content and dry matter rate of plantlets in vitro, with the increasing of day-night temperature difference and then was highest one under the treatment that day-night temperature difference was 15℃.Overall, the morphological growth of Oncidium plantlets in vitro was best under the treatment that the day-night temperature difference was 3℃, the root grew better under the treatment that the day-night temperature difference was 12℃ than others, and when the day-nigh difference was 15℃, it was the best treatment for benefiting the dry material accumulation and the synthesis of chlorophyll.%以文心兰‘柠檬黄'试管苗为材料,探讨了不同昼夜温差对其生长的影响.结果表明:昼夜温差3℃处理下文心兰试管苗的株高、叶长、总鲜重、地上部鲜重、总干重、地上部干重均达到最大值,且与其他处理差异性显著.昼夜温差12℃时文心兰试管苗根数、最大根长、地下部鲜重及干重最大,且在此处理下可溶性糖含量和根系活力也达到最大值.叶绿素a、叶绿素b、总叶绿素含量和干物率均随着昼夜温差的不断增大呈现出增大的趋势,且在昼夜温差15℃时达到最大值.综上分析,在昼夜温差3℃时最有利于促进文心兰试管苗的形态生长,昼夜温差12℃时最有利于根部的生长,昼夜温差15℃时最有利于干物质的积累和叶绿素的合成.