This experiment utilized Dongnong423, Dongnong425, Songjing6 and Songjing9 as the tested varieties, the effect of different nitrogen levels on the glutamine synthetase(GS) activity in grains and the yield of rice was studied. The results showed that dynamics of GS activities gradually declined since the heading, the effect nitrogen application could increase the GS activities, when the best GS activity apply nitrogen was 150 kg·hm~(-2) for Dongnong425 and Songjing9, but the treatment of 100 kg·hm~(-2) was the best for Dongnong423 and Songjing6. To a certain extent, the yield of rice increased with the increasing of nitrogenous fertilizer. N150 treatment was the best for Dongnong423, Dongnong425 and Songjing6 to ensure a high yield, while N200 was a prime treatment for Songjing9. The GS activities in grains were positively related to the yield. The GS activities at 7, 35 d after heading showed significantly positive correlation with the yield.%以东农423、东农425、松粳6号和松粳9号4个水稻品种为试验材料,研究了不同氮素处理对水稻籽粒谷氨酰胺合成酶活性及产量的影响.结果表明,籽粒谷氨酰胺合成酶活性在抽穗后呈下降趋势,适量施氮对谷氨酰胺合成酶活性有明显的促进作用;对4个品种来说,东农425和松粳9号以N150处理下有较高的酶活性,东农423和松粳6号则以N100处理为最佳;在一定范围内,籽粒产量随施氮量的增加而增加,对东农423、东农425和松粳6号而言,N150是保证高产最佳施肥量;松粳9号以N200处理为最佳.籽粒谷氨酰胺合成酶活性与籽粒产量呈正相关关系,其中抽穗后7,35 d酶活性与产量呈极显著正相关.
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