番茄抗叶霉病基因Cf-10和Cf-16的遗传分析及SSR标记

摘要

以Cf-10和Cf-16基因对东北三省分离鉴定的8个番茄叶霉病病原菌生理小种的抗性进行了评价,表明Cf-10和Cf-16基因对分化出的生理小种具有良好的抗性.分别对07881 ×08HN30(含Cf-10)和07880×08HN34(含Cf-16)构建的F1、F2代分离群体进行了遗传分析,并对F2群体进行了SSR标记.遗传分析表明,08HN30和08HN34的抗性均由一对显性单基因控制,从341对SSR引物中筛选出2个与Cf-10基因连锁的标记LEtaa001和LEaat003,遗传距离分别为9.7和22.9 cM；1个与Cf-16基因连锁的标记Tom144-145,遗传距离为10.4 cM.上述研究结果为抗源基因在番茄抗叶霉病育种中的应用及进一步精细定位Cf-10和Cf-16基因奠定了基础.%Tomato Cf-10 and Cf-16 gene were resistant to eight prevalent physiological races of C. Fulvum present in three northeastern provinces of China by virulence tests. F1 and F2 populations were developed from 07881 ×08HN30 and 07880 × 08HN34. The inheritance analysis indicated that leaf mould resistance gene Cf-10 and Cf-16 were single dominant genes, respectively. Total 341 pair of SSR primers were used to identify molecular markers linked to Cf-10 and Cf-16 gene, two polymorphic markers LEtaa001and LEaat003 were identified to be linked to Cf-10 in 08HN30 with the genetic distances of 9.7 and 22.9 cM, respectively, while a SSR marker Tom144-145 located on chromosome 11 were linked to Cf-16 resistant gene with the genetic distances of 10.4 cM.