目的 观察大鼠动脉血管平滑肌细胞株A7r5在高浓度葡萄糖作用下的增殖变化以及不同浓度的枸杞多糖(LBP)对该作用的影响.方法 将A7r5细胞培养48h后随机分组:对照组(葡萄糖浓度5mM)、高糖组(葡萄糖浓度10、15、20、25、30、40mM)、高糖(25mM)+LBP 60μg·mL-1组、高糖(25mM)+LBP 80μg·mL-1组、高糖(25mM)+LBP 100μg·mL-1组,以MTS孵育A7r5后,经酶联免疫检测仪测定各组的吸光度值.结果 ①与对照组(1.331±0.097)相比,高糖组吸光度值均明显升高,且以葡萄糖浓度为25mM组(2.485±0.229)增高为甚,与对照组相比差异均有统计学意义(P＜0.01).②高糖(葡萄糖浓度25mM)诱导的A7r5细胞增殖在48～72h达到高峰.③与高糖组相比,中、高浓度LBP 组对A7r5细胞增殖有抑制作用,其差异均有统计学意义(P＜0.01).结论 高浓度葡萄糖可促进A7r5细胞增殖,中、高浓度LBP可抑制高糖诱导的A7r5增殖.%Objective To observe the proliferation of A7r5 cells when high concentration glucose present and the effect of different concentrations of Lycium barbarum polysaccharides ( LBP) on the course. Methods Groups; Control (glucose 5mM), high concentration glucose groups (including 10, 15,20,25,30 and 40mM), glucose (25mM) + LBP60 μg ? mL-1, glucose (25mM) + LBP80μg ? mL-1, glucose (25mM) + LBP100μg ? mL-1 . After co -incubating of A7r5 and MTS, OD reading of each group was measured by enzyme immunoassay detecting instrument. Results (1) The OD readings in high glucose groups were all significantly increased, especially in 25mM group (2. 485 ±0. 229) , compared with controls ( 1. 331 ±0. 097) ( either P <0. 01) . (2) A7r5 proliferation reached its peak when incubated together with glucose(25mM) for 48 -72 hours. (3) Compared with 25mM glucose group ,the OD readings of LBP 80μg ? mL-1 and 100μg ? mL-1 groups were significantly decreased( either P <0. 01) . Conclusion High concentration glucose accelerates A7r5 proliferating, and LBP suppresses the proliferation of A7r5 induced by high concentration glucose.
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