目的 采用大鼠自由饮用不同低浓度的酒精饮料的造模方法,建立与人体慢性酒精中毒类似的简便、理想的大鼠慢性酒精中毒自由饮模型.方法 将成年Wistar雄性大鼠40只随机分成对照组、6％酒精组、8％酒精组和12％酒精组,每组各10只.对照组大鼠给予自来水,24 h自由饮用.模型组大鼠每天自由饮用各浓度的酒精饮料,不给予水.造模4个月后通过气相色谱法测定血酒精浓度,采用光学显微镜及电子显微镜分别观察酒精中毒后大鼠脑部的病理变化.结果 (1)造模4个月后,模型组大鼠血中酒精浓度在150～200mg/100ml.(2)造模后透射电镜下模型组额叶、小脑、海马突触间隙厚度变薄,突触数量减少,突触小泡数量增多,突触间隙变窄,突触厚膜致密物电子密度降低；光镜下仅有小脑及海马有病理改变.结论 自由饮用低浓度酒精饮料4个月可以建立慢性酒精中毒大鼠自由饮模型.%Objective To investigate the drinks of different alcohol concentrations to rats, and to create the simple and ideal rat's chronic alcoholism model similar to mankind. Methods Forty Wistar male rats were randomly into comparison group, 6% alcohol group, 8% alcohol group and 12% alcohol group averagely. The model rat groups were provided with alcohol of different concentrations without water. After 4 month, gas chromatography was adopted to determine alcohol concentration in rat's blood while optical microscope and electron microscope were applied to inspect rat brain's pathological change after alcoholism. Results (1) After feeding the model groups with alcohol for 4 months, all examined rat 's alcohol concentration in blood was around 150~200mg/100ml. (2) After 4 months, the interval thickness of frontal lobe, epencephal and hippocampal synapses reduced while the quantity of synapses reduced with more bubbles in synapses, narrower space between synapses and lower electron density in synapses dense granules under the electron microscope; only the epencephal and hippocampus suffered pathological change under the optical microscope. Conclusions The four month's drinking drinks of low alcohol concentration is able to establish low chronic alcoholism rat model.
展开▼
