nano-TRFIA法联合检测健康人血清Galectin-3及其与常规TRFIA法的比较

摘要

Objecti ve: To establish nanomagicbeabs sorting-TRFIA tandem analysis method for the detection of serum Galectin-3 for healthy people and comparison with the conventional time resolved fluoroimmunoassay method. Methods:Serum levels of Galectin-3 were measured by application of nanomagicbeabs sorting with monoclonal anti-human Galectin-3 labeled antibody and tandem TRFIA in healthy volunteers. Results:Serum levels of Galectin-3 in healthy volunteers by nano-TRFIA was (0.380 3±0.312 9) ng/mL, and that by conventional TRFIA was (0.290 1±0.278 3) ng/mL. The value of Galectin-3 by nano-TRFIA can reach picogram level. There was a significant difference between nano-TRFIA and conventional TRFIA on detection sensitivity (P<0.05). Conclusion: Nanomagicbeabs sorting-TRFIA tandem analysis method has the ability to use repeatly of serum specimens and to improve the detection sensitivity of serum Galectin-3.%目的：研究通过建立纳米磁珠分选-时间分辨免疫荧光联合检测法(nanomagicbeabs sorting-time resolved fluoroimmunoassay，nano-TRFIA)对健康人血清半乳糖凝集素-3(Galectin-3)进行检测并与常规时间分辨免疫荧光法(time resolved fluoroimmunoassay，TRFIA)比较。方法：采用Galectin-3抗体标记的纳米磁珠对20例健康志愿者的外周血血清进行分选，并串联时间分辨免疫荧光进行检测。结果：使用nano-TRFIA检测出的Galectin-3血清质量浓度为(0.3803±0.3129) ng/mL，而使用常规TRFIA检测出的Galectin-3血清质量浓度为(0.2901±0.2783) ng/mL；nano-TRFIA检出值可达到皮克水平，与常规TRFIA比较，两者的检测灵敏度差异有统计学意义(P<0.05)。结论：nano-TRFIA够实现对血清标本的重复使用，并能提高对血清Galectin-3的检测灵敏度。