Objective To produce the antiserum of chicken brain derived neuron growth inhibitor collapsin 1. To detect its ability to neutralize the collapsing activity of collapsin 1. Methods The c myc epitope tagged entire sequence of collapsin 1 was amplified by PCR and expression cloned. Rabbits were immunized with the c myc tagged collapsin 1 to produce antiserum of collapsin 1. Its ability to neutralize the collapsing activity of collapsin 1 was observed in dorsal root ganglia (DRG) growth cone. Results Collapsin 1 could induce the collapse of DRG growth cone. The collapsing activity of collapsin 1 could be neutralized by the antiserum of collapsin 1. Conclusion We produced the antibody of a neuron growth inhibitor collapsin 1 that could block its inhibiting function.
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机译:Neural activity in the periaqueductal gray and other specific subcortical structures is enhanced when a selective serotonin reuptake inhibitor selectively prevents seizure-induced sudden death in the DBA/1 mouse model of sudden unexpected death in epilepsy
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