'太岁'生物学组分的研究

摘要

"太岁"是一种存在于地球上分类地位尚不清楚的生物体.对实验室保藏的7种不同的"太岁"样品进行了生物学组分的定性定量分析,旨在科学地了解"太岁"的组成成分,同时为"太岁"有效成分的开发利用和分类鉴定提供理论依据.对实验室保藏的7种"太岁"样品做生物组分及含量分析,具体包括:苯酚硫酸法测粗多糖含量、凯氏定氮法测粗蛋白含量、索氏提取法测粗脂肪含量、钒钼酸显色法测核酸含量、妁烧重量法测粗灰分含量、恒温干燥法测水分含量、原子吸收法测微量元素含量.实验结果表明.7种"太岁"样品含水量均较高,除LNUT006含水量73.58%外,其余"太岁"样品均达到90%以上;"太岁"样品中粗多糖含量在8.97-46.57 mg/g之间,其中LNUT006多糖含量明显高于其他"太岁"样品;粗蛋白含量在20.35～184.18 mg/g之间,其中LNUT006蛋白含量明显高于其他"太岁"样品;"太岁"样品中净核酸含量在2.053～35.1286μg/g之间;灰分含量在42.93～246.4 mg/g之间;"太岁"样品钙、铁含量较高,分别为2907.09μg/g和3929.09μg/g,铅、镉含量最低.首次对未知生物"太岁"样品的组成成分进行初步系统的研究,为明确其组分和对其进一步的开发利用均有很重要的意义.%"Tai sui" (Ts) is an organism of yet unclear taxonomic position exists on the earth.Seven kinds of Ts samples preserved in the lab were studied for their bio-components analyses qualitatively and quantitatively; wishing scientifically understand bio-component of Ts to provide theoretical foundation for the development, uses, and taxonomic characterization of the organism.Phenol suffuric acid method was used to analyse polysaccharide content, Kjeldahl method for crude protein content, Soxhlet method for crude fat content, vanado-molybdic acid coloration method for total phosphorus content, burning weight method for ash content, constant temperature drying method for water content, and AAS method for trace elements.The results showed that the Ts samples had high water content (over 90％ ) except of LNUT006 (73.58％).The polysaccharide content was between 8.97 ～ 46.57 mg/g except of LNUT006, which polysaccharide component was significantly higher than others.The crude protein content was between 20.35 ～ 184.18 mg/g, among which the LNUT006 protein was significantly higher than others.The net nucleic acid content was between 2.053 ～ 35.128 6 μg/g.The crude ash content was between 42.93 ～ 246.4 mg/g.Calcium and iron content was high up to 2 907.09 ～ 3 929.09 μg/g, lead and cadmium content was the lowest.It was for the first time to study the unknown organism "tai sui" samples on their components initially and systematically.It is preliminary and has a great significance on clarification of their constituents and their further exploitation.