The Polymorphism of the HLA-DRB1 Locus of the Guangxi Zhuang People and Its Comparison with the Main Surrounding Ethnic Groups

摘要

To explore genetically the origin, migration and relationship of the Zhuang people in Guangxi province with the main surrounding Chinese and the ethnic groups in Southeast Asia, the distributions of the HLA-DRB1 locus among the populations in these regions were genotyped by polymerase chain reaction—sequence specific primer (PCR-SSP) technique among 265, healthy, randomly selected and unrelated Zhuang adults, aged 17-32, whose families had lived in Guangxi province for more than 3 generations. Every allelic frequency was comparatively analyzed with that of some main Chinese and Southeast Asia populations. It was found that 13 HLA-DRB1 alleles were detected among these 265 selected individuals, but other 13 alleles failed to be discovered by using the 23 pairs of primers designed in the present study. The most common alleles were HLA-DRB1*1501, HLA-DRB1*1601/2 and HLA-DRB1*1401/4 with the allele frequencies of 0.291, 0.198 and 0.106 respectively. Although alleles of HLA-DR1*0701/2, HLA-DRB1*08 and HLA-DRB1*1306/1406 could be detected also, but their frequencies were rather very low. The overall allele distribution profile on HLA-DRB1 of Zhuangs was much closed dramatically to those of Buyis, Northeast Thais, Dai Lues, Southern Hans and Singapore Chinese, but was more far away from that of northern Hans and Hanai Kinhs, as compared to the representative ethnic groups in Southeast Asia. In addition, the blank of HLA-DRB1*1502 was a characteristic allele for Zhuangs was commonly detected in Southeast Asia, especially in Thais. It is concluded that a very close genetic relationship exists between Zhuangs and Buyis on the basis of their extremely similar background on HLA-DRB1 locus.