Macroporous and Antibacterial Hydrogels Enabled by Incorporation of Mg-Cu Alloy Particles for Accelerating Skin Wound Healing

摘要

Repair of severe skin tissue injury remains a great challenge and wound infection is still a formidable problem.In this study,new macroporous and antibacterial gelatin/alginate(SAG)-based hydrogels for wound repair were designed and developed based on in-situ gas foaming method and ion release strategy as a result of Mg-Cu particles degradation in the hydrogel matrix.The addition of Mg-Cu particles decreased the storage modulus of SAG,maintained its mechanical resilience and enhanced its water-absorbing capability.Moreover,the water vapor transmission rate of SAG added with 2 wt.%Mg-Cu(SAG-2 MC)was 124%of that of medical gauze and 804%of commercial Tegaderm^(TM)film dressing.The bacterial inhibition rates of SAG-2 MC against S.aureus.E.coli and P.aeruginosa reached 99.9%±0.1%,98.7%±1.2%and 98.0%±0.7%,respectively,significantly greater than those of the SAG hydrogel and Mg particle-modified hydrogels.In addition,SAG-2 MC hydrogel was biocompatible and promoted cell migration.In vivo experiment results indicated that SAG-2 MC significantly accelerated the skin wound healing in murine model as demonstrated by higher epidermis thickness,more collagen deposition and enhanced angiogenesis compared with SAG-OMC,SAG-2 M and TegadermTM film.In summary,Mg-Cu particles have great potential to modulate the physiochemical and biological properties of SAG hydrogels.Mg-Cu particle-modified SAG hydrogels reveal significant promise in the treatment of severe skin wound or other soft tissue lesions.