表皮生长因子基因多态性与结直肠癌发生及其临床病理特征之间的关系

摘要

Objective To study the relationship between epidermal growth factor ( EGF ) G6IA polymorphism and the susceptibility as well as clinicopathological characteristcs of colorectal cancer ( CRC ). Methods EGF G61A polymorphism was detected by polymerase chain reaction - restriction fragment length polymorphism ( PCR - RFLP ) among 218 CRC patients and 200 health subjects. The pathological data were obtained by reviewing of patients' hospitalized documentation. Statistical software SPSS 11. 0( SPSS, Inc. )was used to analyze these data. Results The distribution frequencies of GG genotype and G allele were 56. 4％ ,51. 0％ and 76. 6％ ,73. 3％ in CRC patients and health controls, respectively. The distribution frequencies had no significant difference ( P ＞ 0. 05 ). But the patients with GG genotype or G allele were related to Duke C or D stage and the occurrence of distant metastasis. Conclusion EGF G61A polymorphism showed no susceptible to CRC , but GG genotype and G allele probably were close related to clinical progression of CRC patients.%目的 研究表皮生长因子(EGF)G61A多态性和结直肠癌发生及其临床病理特征之间的关系.方法 采用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)方法检测了218例结直肠癌患者和200例健康体检者EGF61位点基因型,分析基因型和等位基因频率在两组间分布差异及其和肿瘤临床特点之间的关系.结果 EGF61位点GG基因型和G等位基因在结直肠癌患者和健康体检者中分布分别为56.4%,51.0%和76.6%,73.3%,在两组间分布均无显著性差异(P>0.05).但GG基因型和G等位基因均和C和D期肿瘤(Duke分期)、伴远端转移有关(P<0.05).结论 EGF G61A多态性和结直肠癌发生无关,但GG基因型可能与更晚的临床分期及易发生远端转移相关.