PARP-1基因多态性与临夏回族自治州回族人群胃癌易感性的关联研究

摘要

目的 探讨聚腺苷二磷酸核糖聚合酶-1(PARP -1)Va1762Ala的单核苷酸多态与临夏回族自治州回族人群胃癌易感性的关系.方法 采用病例-对照研究设计,研究对象为临夏回族自治州居民(20岁以上,居住满20年以上,3代无族外通婚).分两组:胃癌组200例,均经组织学确诊.对照组210例,按年龄、性别进行匹配的无肿瘤史的健康回族志愿者.抽提血液DNA,采用聚合酶链反应-限制性片段长度多态性方法(PCR - RFLP)进行基因分型.使用ELISA( enzyme - linkedimmunosorbent assay)法检测血浆中H.pylori的IgG抗体,以检测研究对象是否有H.pylori感染.用SPSS 13.0统计软件分析试验结果.结果 PARP -1 762Val/Ala+ Ala/Ala在胃癌组显著增高(OR:2.158,95％ CI:1.404～3.315,P=0.020).交互作用分析显示:家族史阳性的Val/Ala+Ala/Ala基因型患胃癌的风险是家族史阴性的Val/Val基因型的4.125倍(OR:4.125,95％ CI:2.117～8.038,P =0.000)；食腌菜的Val/Ala+Ala/Ala基因型患胃癌的风险是不食腌菜的VaL/Val基因型的3.814倍(OR:3.814,95％ CI:2.150～6.766,P=0.000)；H.pylori感染阳性的Val/Ala+Ala/Ala基因型患胃癌的风险是H.pylori感染阴性Val/Val基因型的4.085倍(OR:4.085,95％ CI:2.197 ～7.597,P=0.000).结论 PARP -1 Va1762Ala单核苷酸多态与临夏回族自治州回族人群胃癌易感性增高相关.PARP -1 Val/Ala+ Ala/Ala基因型分别与家族史、食腌菜、H.pylori感染在胃癌的发病风险中存在着加乘交互效应.%Objective To explore the correlation between nucleotide polymorphisms in polymerase - 1 ( PARP - 1) Val762Ala and the susceptibility of gastric cancer in Hui ethnic group of Linxia Hui Autonomous Prefecture. Methods A case - control study was performed. All investigated objects were Hui ethnic population of Linxia Hui Autonomous Prefecture that included 2 groups; gastric cancer group(200 gastric cancer patients) ,and control group (210 controls, frequency - matched for age and sex). Polymerase chain reaction - restricted fragments length polymorphism ( PCR - RFLP) method was used to detect genotypes of PARP - lVal762Ala polymorphisms. ELASA( enzyme - linked immunosorbent assay) method was used to identify IgG to H. Pylori. The results were analyzed by SPSS13. 0. Results PARP - 1762Val/Ala + Ala/Ala genotypes were overrepresented in the gastric cancer group ( OR:2. 158,95% CI: 1. 404 -3. 315 ,P =0.020). Interaction analysis showed that: Val/Ala + Ala/Ala genotypes carriers with positive family history of gastric cancer had 4. 125 fold increased risk for developing gastric cancer compared with Val/ Val genotypes carriers with negative family history of gastric cancer (0R:4. 125,95%CI:2. 117 - 8. 038 ,P =0. 000) ; Val/Ala + Ala /Ala genotypes carriers with eating pickle vegetables had 3. 814 fold increased risk for developing gastric cancer compared with Val/Val genotypes carriers with not -eating pickle vegetables (OR; 3. 814 ,95%CI:2. 150 -6.766, P =0.000). Val/Ala + Ala/Ala genotypes carriers with positive H. Pylori infection had 4.085 fold increased risk for developing gastric cancer compared with Val/Val genotypes carriers with negative H. Pylori infection( OR ;4. 085 ,95% CI; 2. 197 - 7.597, P = 0.000). Conclusion PARP - lVal762Ala contributed to the increased susceptibility of gastric cancer in Hui ethnic group in Linxia Hui Autonomous Prefecture. Multiplicative interactions lied in between PARP - 1762Val/Ala + Ala/Ala genotypes and positive family history of gastric cancer, eating pickle vegetables, H. Pylori infection in risk of developing gastric cancer.