银胶和银岛膜上胸腺嘧啶的表面增强拉曼光谱对比研究

摘要

Surface-enhanced Raman scattering (SERS) spectra of thymine obtained on silver colloids and island films were studied comparatively. The spectroscopic characterization showed that the molecule configuration of thymine hydrolyzed ion compound was transformed from keto-amino to enol-amino after adsorption. On either silver surface, the N(3)-deprotonated tautomer was favorable. Thymine adsorbed on the silver colloids surfaces as enolate through the 0(7) and N(3) atoms while through the 0(8) and N(3) atoms in the case of silver island films surfaces.%利用表面增强拉曼光谱对胸腺嘧啶在银胶和银岛膜上的吸附状态进行了对比研究.光谱分析表明,胸腺嘧啶吸附在银胶和银岛膜表面后其分子结构发生了互变异构,由原来的酮式结构变成了烯醇式结构.在银胶和银岛膜表面胸腺嘧啶主要以N(3)去质子化异构体的形式存在.胸腺嘧啶通过O(7)和N(3)倾斜地吸附在银胶颗粒表面,通过O(8)和N(3)倾斜地吸附在银岛膜表面.