间充质干细胞诱导体外分化来的初始T细胞表型变化的实验研究

摘要

目的 将体外由CD+34细胞分化而来的初始T细胞与异基因骨髓间充质干细胞(MSC)共孵育,观察初始T细胞表型的变化.方法 传3代以上的MSC与脐血CD+34细胞分化的初始T细胞共孵育1周.用流式细胞仪检测其表型变化.结果 与对照组相比,CD+8细胞明显增加[共孵育组(35.9±6.3)%,单纯初始T细胞培养组(18.4±4.5)%],且CD;CD;细胞明显增加[共孵育组(27.6±2.8)%,单纯初始T细胞培养组(15.2±3.1)%].结论 骨髓MSC在体外与异基因初始T淋巴细胞共孵育使CD+8初始T细胞表达增加,这种变化町能与其诱导免疫耐受相关.%Objective Donor derived naive T cells initiated GVHD by contacting with,mesenchymal stem cells(MSC)have been used to prevent or treat graft-versus-host disease(GVHD).although we stiff puzzle about its mechanisms.Observe the effect of MSC on phenotypes of Naive T cell to study the mechanism of MSC immunomodulation. Methods After 3 passages, MSC Was incubated with Naive T cell differentiated from COrd blood CD+34 cells in vitro.Then the variances of naive T cell phenotypes were analyzed by flow cytometric.Results CD+8 T cells were relatively increased after 7 days co-culture with allogenic MSC when compared to control:(35.9±6.3)%VS(18.4±4.5)%.CD+8 CD+3 cells also showed the same trend (27.6±2.8)%vs(15.2±3.1)%.Conclusion MSC may partly reduce the incidence of GVHD by increase of CD+8 naive T cell.The result may provide new clue to explain immunoregulatory mechanism of MSC.