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七龙天胶囊质量标准实验研究

     

摘要

Applying the method of chromatography of thin-layer(TLC)made the qualitative identification of pberetima in Qilongtian capsules. The results show that on the corresponding position on TLC,pberetima with the contrasted drug has the similar color spot and without any interfere with negative contrast. Also applying the method of chromatography of high performance liquid (HPLC)identified the quality mass of notoginsenoside R1 ,ginsenoside Rg1 ,ginsenoside Rb1 and salidroside in qilongtian capsules in the prescription. The results show the HPLC linear ranges of notoginsenoside R1 ,ginsenoside Rg1 ,ginsenoside Rb1 and salidroside were 0.178—3.560μg, 0.539—10.780μg,0.430—8.600μg,0.206—4.120μg,respectively,and r were 0.999 7,0.9997,0.999 8,1.000 0(n=6)respec-tively.The average recoveries were 97.82%(RSD of 1.28%)for notoginsenoside R1 ,97.07%(RSD of 0.82%)for ginsenoside Rg1 , 97.92%(RSD of 1.26%)for ginsenoside Rb1 ,96.58%(RSD of 1.45%)for salidroside respectively. The TLC and HPLC method is proved to be sensitive,accurate,repeatable,and can be used for quality control of the Qilongtian capsules.%采用薄层色谱法(TLC)对七龙天胶囊中的地龙进行定性鉴别,结果表明,地龙对照药材在与供试品的薄层色谱相应位置上,有相同颜色斑点,阴性对照无干扰.另采用高效液相色谱法(HPLC)测定处方中三七皂苷R1、人参皂苷Rg1和Rb1及红景天苷的质量分数,结果显示,三七皂苷 R1在0.178~3.560μg、人参皂苷 Rb1在0.430~8.600μg、人参皂苷Rg1在0.539~10.780μg、红景天苷在0.206~4.120μg范围内与峰面积值呈良好线性关系(r分别为0.9997,0.9998,0.9997,1.0000,n =6).平均加样回收分别为97.82%(RSD=1.28%),97.92%(RSD=1.62%),97.07%(RSD=0.82%),96.58%(RSD=1.45%).TLC法及HPLC法操作简便、准确、重复性好,可对七龙天胶囊进行质量控制.

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