膀胱癌组织中白细胞介素-12B基因启动子区甲基化的变化及其意义

摘要

目的 研究膀胱癌组织中白细胞介素-12B基因(IL-12B)启动子区甲基化的变化及其临床意义.方法 应用甲基化特异性聚合酶链反应、Western-blot检测膀胱癌及癌旁组织78例,比较IL-12B基因启动子区甲基化及其蛋白质与膀胱癌发生发展的关系.结果 (1)膀胱癌组织IL-12B启动子区甲基化产物明显多于癌旁组织,差异有统计学意义(x2=14.8,P=0.001)； (2)膀胱癌组织和癌旁组织IL-12B启动子区甲基化产物的PMR水平差异有统计学意义(t=3.42,P=0.001)； (3)膀胱癌组织IL-12B蛋白的表达水平显著低于癌旁组织(t=2.832,P=0.012).结论 IL-12B基因启动子区甲基化及其编码蛋白质的失活与膀胱癌的发生相关.%Objective To analyze the clinical implications of methylation of the interleukin-12B gene (IL-12B) on promoter area in bladder cancer tissues.Methods The tissues of 78 cases of bladder cancer and para-carcinoma tissues were studied.The expression of methylation of IL-12B gene on promoter area was detected by polymerase chain reaction (PCR).The protein expression of IL-12B was determined by western blot analysis.Results (1) The product of methylation of IL-12B gene on promoter area of the bladder cancer tissues was significantly higher than that of the para-carcinoma tissues (the chi-square =14.8,P=0.001).(2) The PMR level of IL-12B gene in bladder cancer and para-carcinoma tissues was statistically significant (t =3.42,P =0.001) (3) The IL-12B protein expression level of bladder cancer tissues was significantly less than that of para-carcinoma tissues (t =2.832,P =0.012).Conclusion The methylation of IL-12B gene on promoter area and the inactivation of IL-12B protein are significantly related to bladder cancer.