采用均匀设计法优化PCR复合扩增的实验研究

摘要

Objective To investigate the application of uniform design in optimizing multi-PCR (polymerase chain reaction) conditions. Method Four factors including MgCl2, dNTPs, DNA template and cycle number, were tested using uniform design U6 (64). Result DNA bands were clear, and a suitable multi-PCR system for 5 Y-SNPs (M130, M175, M174, M231 and M45) was established. Conclusion The optimization of multiplex system can be used in forensic medical examination.%目的 探索均匀设计法在优化PCR复合扩增条件中的应用.方法 采用均匀设计方法,对影响PCR扩增体系的MgCl2、dNTP、模板DNA和扩增循环次数等因素进行了U6 (64)优化.结果 获得5个条带清晰的目的DNA扩增产物,建立了适用于5个Y-SNP位点(M130、M175、M174、M231、M45)的复合扩增反应体系.结论 该优化复合扩增体系可用于法医物证学实验研究.