新型肽 VIP-TAT 对 NMDA 诱导的大鼠视网膜损伤的神经保护作用

摘要

Aim:The aim is to observe the protective effects of VIP-TAT against NMDA-induced reti-nal damage in rats.Methods:Retinal damage was induced by injecting NMDA (40 nmol in a 2 μL sa-line solution)intravitreally.Different dosages of VIP-TAT were administered in treatment groups.And normal saline were administered in NMDA group.The visual behavior of rats were evaluated using the black-white box.Electroretinogram was employed to evaluate changes in visual function.HE staining was used to evaluate retinal histology in different groups.Results:Visual behavior detection showed there was no significant difference in time spent in the black chamber among normal group,NMDA group and NM-DA +VIP-TAT treatment groups (P >0.05 ).NMDA reduced the amplitudes of b-wave and PhNR, which was attenuated by VIP-TAT administration.HE-staining showed that VIP-TAT treatment at the concentration of 1nmol /L significantly maintained the thickness of rat retina and increased the cell num-ber in ganglion cell layer (GCL)in NMDA-induced rat glaucoma model.Conclusion:In NMDA-induced rat glaucoma model,novel peptide VIP-TAT exerts a protective effect of RGCs in the retina.%目的：研究新型肽 VIP-TAT（血管活性肠肽重组肽）对 NMDA（N-甲基-D-天门冬氨酸）诱导的大鼠视网膜损伤的神经保护作用．方法：向 SD 大鼠双眼玻璃体腔注射40nmol NMDA （2μL）建立视网膜损伤模型，治疗组向玻璃体腔注射2μL 不同浓度（10 pmol／L、1 nmol／L、100 nmol／L、10μmol／L）的 VIP-TAT，NMDA 组用等体积生理盐水替代，正常对照组不作任何处理．分别于术后7天用黑白箱装置对其行为学进行检测，观察每组大鼠术后视觉行为的变化；采用视网膜电图评估每组大鼠视功能的差异；石蜡切片 HE 染色比较各组大鼠视网膜形态学的差异．结果：行为学检测显示，各组大鼠在暗室停留时间的无统计学差异（P ＞0.05）；视网膜电图检测结果表明浓度为1 nmol／L的 VIP-TAT 能够显著提高因 NMDA 损伤而降低的 b 波与明视负向反应（PhNR）的振幅；HE 染色显示：在NMDA 诱导的青光眼模型中，浓度为1 nmol／L 的 VIP-TAT 能有效增加 NMDA 诱导的青光眼模型视中视网膜神经节细胞的存活数目．结论：NMDA 诱导的 SD 大鼠青光眼模型中，VIP-TAT 对视网膜神经节细胞有保护作用．