西藏砂生槐丛生芽的诱导

摘要

以西藏砂生槐无菌实生苗为材料，研究了不同外植体、基本培养基、细胞分裂素种类及浓度、pH对其丛生芽诱导的影响。结果表明：以子叶节为外植体，接种于pH 6.0～6.5的MS+6-BA 2.0 mg/L+NAA0.2 mg/L培养基上，丛生芽的诱导效果最好。%Hypocotyl explants induced adventitious buds from Sophora moorcroftiana seedling by adopting shoot tips, stems and cotyledon nodes. Different explants, basal substrates, kinds and concentrations of cytokinin and pH value were involved. The results showed lhat the induction effects of Multiple Shoots were the best in the basic medium at the conditions of pll 6.0--6.5 with MS + 6-BA 2. 0 mg/L+ NAA 0.2 mg/L.