选择来自于亚蔬—世界蔬菜研究与发展中心和国家作物种质资源库20个对辣椒疫病具有不同抗性的辣椒品系，采用灌根法人工接种辣椒疫霉菌3号生理小种，根据不同辣椒品系的病情指数，将20个品系划分为免疫（ I）、高抗（ HR）、抗（ R）、中抗（ MR）和感（ S）5种抗病类型。采用常规方法测定辣椒叶片6种防御酶活性和根际8种土壤酶活性，判断各项指标与辣椒抗疫病特性的相关性。结果表明：叶片防御酶中苯丙氨酸解氨酶、多酚氧化酶、β⁃1，3⁃葡聚糖酶活性与植株抗病性极显著正相关，过氧化物酶活性与植株抗病性呈显著正相关，根际土壤酶中多酚氧化酶、过氧化氢酶、脲酶、蛋白酶活性与植株抗病性显著正相关。叶片中苯丙氨酸解氨酶、多酚氧化酶、β⁃1，3⁃葡聚糖酶活性可作为辣椒抗病种质资源筛选的判断依据，与植株抗病性相关的土壤酶可以作为土壤微环境调控的依据。%Twenty lines of pepper with different phytophthora⁃resistance were observed from AVRDC and Institute of Crop Sciences, CAAS. Race 3 with Phytophthora capsici was artificially inoculated by root⁃irrigation and according to disease index, the 20 lines were divided into 5 phytophthora⁃re⁃sistant types, immunity ( I) , high resistance( HR) , resistance( R) , moderate resistance( MR) and susceptibility ( S) . By conventional methods, activities of 6 defense enzymes in leaf and 8 soil en⁃zymes in rhizosphere were measured to analyze the correlation of different indices with pepper Phyto⁃phthora⁃resistance. Activities of PAL, PPO andβ⁃1,3⁃glucanase in leaf defense enzymes had signif⁃icantly positive correlation with resistance. Activities of POD were positively correlated with resist⁃ance. Meanwhile, resistance was positively correlated with activities of PPO, CAT, Urease and Pro⁃tease in rhizosphere soil enzymes. Activities of PAL, PPO and β⁃1,3⁃glucanase in leaf could be used to identify the resistance of pepper. Soil enzymes related to phytophthora⁃resistance could be used as basis for regulating soil environment.
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