经皮注射医用臭氧治疗兔VX2移植瘤的实验研究

摘要

目的 观察臭氧局部注射治疗实体肿瘤的安全性、对肝肾功能的影响及所致的组织病理学改变.方法 建立兔VX2移植瘤模型24只,分为三组,A、B组均为9只,C组6只(假手术组).向A、B组瘤内分别注入40 μg/ml和70 μg/ml浓度臭氧,并于术前1d、术后1、3d抽静脉血.术后3d处死动物,取病理标本.观察术后动物生命体征、并发症及大体标本的组织学变化.结果 实验动物术后24 h内均出现活动减少、纳差,对刺激反应差,精神萎靡,24 h后恢复正常.A、B组各有1只荷瘤兔于术中或术后1h内死亡.术前1d,术后1、3d各组血清丙氨酸转氨酶、天冬氨酸转氨酶及肌酐比较无明显差异.肉眼及光镜下见A、B组坏死区大体标本无明显差异.C组镜下可见部分肿瘤细胞突破肌层向深处生长,肿瘤细胞排列紊乱,大量癌巢形成,细胞核大深染,核分裂,异性核.结论 VX2肿瘤内注入浓度为40 μg/ml和70μg/ml的医用臭氧安全、有效.%Objective To evaluate the safety and effectiveness of percutaneous medical ozone injection in treating solid transplanted VX2 tumors in rabbits, and to discuss the histopathological changes cause by ozone injection. Methods Transplanted VX2 tumor model was established in 24 rabbits. The rabbits were randomly divided into three groups. Rabbits in group A (n = 9) received medical ozone (40 μg/ ml) injection of the tumor. Rabbits in group B (n = 9) received medical ozone (70 μg/ml) injection of the tumor. Rabbits in group C (n = 6, used as sham group) received no treatment. The serum AIT, AST and Cr levels were determined at one day before and one, three days after the treatment. All the animals were sacrificed at three days after the treatment. The specimens were collected and sent for pathological examination. After the procedure, careful observation of animal's vital signs, complications was executed, and the pathological findings were recorded. Results After the treatment reduced activity, anorexia, slow in reaction and listlessness were seen in all animals within 24 hours. One rabbit in group A and one rabbit in group B died during the procedure or within one hour after the procedure. The serum AIT, AST and Cr levels determined at one day before and one, three days after the treatment showed no significant difference among the three groups (P > 0.05). Macroscopically and microscopically, the difference in the manifestations of necrosis area was not significant between group A and group B. In group C, microscopic examination revealed that some tumor cells broke through the muscle layer and invaded into the deeper tissue, the tumor cells were arranged in disorder with lots of cancer nests, and the nuclei became larger and hyperchromatic with fragmentation. Conclusion For the treatment of transplanted VX2 tumor in rabbits, percutaneous injection of medical ozone with the concentration of 40 μg/ml or 70 μg/ml is safe and effective.