Arabidopsis Profilin Isoforms, PRF1 and PRF2Show Distinctive Binding Activities and Subcellular Distributions

Feng Wang; Yanping Jing; Zhen Wang; Tonglin Mao; Jozef (S)amaj; Ming Yuan; Haiyun Ren

首页> 外文期刊>植物学报（英文版） >Arabidopsis Profilin Isoforms, PRF1 and PRF2Show Distinctive Binding Activities and Subcellular Distributions

【24h】

Arabidopsis Profilin Isoforms, PRF1 and PRF2Show Distinctive Binding Activities and Subcellular Distributions

机译：拟南芥前体蛋白同工型，PRF1和PRF2显示独特的结合活性和亚细胞分布。

获取原文

获取原文并翻译 | 示例

掌桥外文数据库（机构版） >>

开具论文收录证明 >>

页面导航

著录项
相关主题

机译：Profilin is an actin-binding protein that shows complex effects on the dynamics of the actin cytoskeleton. There are five profilin isoforms in Arabidopsis thaliana L. However, it is still an open question whether these isoforms are functionally different. In the present study, two profilin isoforms from Arabidopsis, PRF1 and PRF2 were fused with green fluorescent protein (GFP) tag and expressed in Escherichia coli and A. thaliana in order to compare their biochemical properties in vitro and their cellular distributions in vivo. Biochemical analysis revealed that fusion proteins of GFP-PRF1 and GFP-PRF2 can bind to poly-L-proline and G-actin showing remarkable differences. GFP-PRF1 has much higher affinities for both poly-L-proline and G-actin compared with GFP-PRF2. Observations of living cells in stable transgsnic A. thaliana lines revealed that 35S::GFP-PRF1 formed a filamentous network, while 35S::GFP-PRF2 formed polygonal meshes. Results from the treatment with latrunculin A and a subsequent recovery experiment indicated that filamentous alignment of GFP-PRF1 was likely associated with actin filaments. However, GFP-PRF2 localized to polygonal meshes resembling the endoplasmic reticulum. Our results provide evidence that Arabidopsis profllin isoforms PRF1 and PRF2 have different biochemical affinities for poly-L-proline and G-actin, and show distinctive Iocalizations in living cells. These data suggest that PRF1 and PRF2 are functionally different isoforms.

著录项

来源
《植物学报（英文版）》 |2009年第2期|113-121|共9页
作者
Feng Wang; Yanping Jing; Zhen Wang; Tonglin Mao; Jozef (S)amaj; Ming Yuan; Haiyun Ren;
展开▼
作者单位

State Key Laboratory of Plant Physiology and Biochemistry China Agricultural University Beijing 100094 China;

Collage of Life Sciences Beijing Normal University Beijing 100875 China;

College of Biological Sciences and Biotechnology Beijing Forestry University Beijing 100083 China;

Institute of Cellular and Molecular Botany Rheinische Friedrich-Wilhelms- University Bonn Department of Plant Cell Biology Kirschallee 1 D-53115 Bonn Germany;

Institute of Plant Genetics and Biotechnology Slovak Academy of Sciences Akademicka 2 SK-95007 Nitra Slovak Republic;

展开▼
收录信息中国科学引文数据库(CSCD);中国科技论文与引文数据库(CSTPCD);
原文格式 PDF
正文语种 chi
中图分类植物学;
关键词
入库时间 2022-08-19 04:30:50

Arabidopsis Profilin Isoforms, PRF1 and PRF2Show Distinctive Binding Activities and Subcellular Distributions

著录项

相关主题

期刊订阅