Cloning and Expression Analysis of Rice Sucrose Transporter Genes OsSUT2M and OsSUT5Z

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Cloning and Expression Analysis of Rice Sucrose Transporter Genes OsSUT2M and OsSUT5Z

机译：水稻蔗糖转运蛋白基因OsSUT2M和OsSUT5Z的克隆和表达分析

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机译：Two sucrose transporter (SUT) cDNAs, OsSUT2M and OsSUT5Z, were isolated from rice (Oryza sativa L.) by reverse transcription polymerase chain reaction (RT-PCR). Sequencing results indicate they are 1 531 bp and 1 635 bp in length including complete open reading frame 1506bp and 1608bp, which encode 502 amino acids and 536 amino acids,respectively. The TopPred program suggested that both sucrose transporter proteins, OsSUT2M and OsSUT5Z, consist of potentially 12 transmembrane domains. Semi-quantitative RT-PCR was carried out to investigate the gene expression patterns of OsSUT2M and OsSUT5Z. In vegetative organs, transcripts of OsSUT2M were higher in source leaf blades than in other organs at the same development stage, whereas transcripts of OsSUT5Z were less traceable in all organs investigated. In reproductive organs, both transcripts of these two genes were high in panicles from the booting stage to 7 days after flowering (DAF) and then sharply declined. The potential physiology functions of these two sucrose transporters are discussed.

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《植物学报（英文版）》 |2008年第1期|62-75|共14页
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作者单位

State Key Laboratory of Plant Genomics Institute of Genetics and Developmental Biology the Chinese Academy of Sciences Beijing 100101 China;

Graduate School of the Chinese Academy of Sciences Beijing 100049 China;

Feed Research institute Chinese Academy of Agricultural Sciences Beijing 100081 China;

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收录信息中国科学引文数据库(CSCD);中国科技论文与引文数据库(CSTPCD);
原文格式 PDF
正文语种 chi
中图分类植物学;
关键词
cDNA cloning; expression pattern; OsSUT2M; OsSUT5Z; rice sucrose transporter.;

机译：cDNA克隆;表达模式OsSUT2M;OsSUT5Z;大米蔗糖转运蛋白。;
入库时间 2024-01-27 11:20:14

Cloning and Expression Analysis of Rice Sucrose Transporter Genes OsSUT2M and OsSUT5Z

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