Phosphorylation of Microtubule-associated Protein SB401 from Solanum berthaultii Regulates Its Effect on Microtubules

Bao-Quan Liu; Lifeng Jin; Lei Zhu; Jiejie Li; Shuli Huang; Ming Yuan

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Phosphorylation of Microtubule-associated Protein SB401 from Solanum berthaultii Regulates Its Effect on Microtubules

机译：茄属植物微管相关蛋白SB401的磷酸化调节其对微管的影响

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机译：We reported previously that the protein SB401 from Solanum berthaultii binds to and bundles both microtubules and F-actin. In the current study, we investigated the regulation of SB401 activity by its phosphorylation. Our experimental results showed that the phosphorylation of SB401 by casein kinase Ⅱ (CKII) downregulates the activities of SB401, namely the bundling of microtubules and enhancement of the polymerization of tubulin. However, phosphorylation of SB401 had no observable effect on its bundling of F-actin. Further investigation using extract of potato pollen indicated that a CKII-like kinase may exist in potato pollen. Antibodies against CKII alpha recognized specifically a major band from the pollen extract and the pollen extract was able to phosphorylate the SB401 protein in vitro. The CKII-like kinase showed a similar ability to downregulate the bundling of microtubules. Our experiments demonstrated that phosphorylation plays an important role in the regulation of SB401 activity. We propose that this phosphorylation may regulate the effects of SB401 on microtubules and the actin cytoskeleton.

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《植物学报（英文版）》 |2009年第3期|235-242|共8页
作者
Bao-Quan Liu; Lifeng Jin; Lei Zhu; Jiejie Li; Shuli Huang; Ming Yuan;
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作者单位

State Key Laboratory of Plant Physiology and Biochemistry Department of Plant Sciences College of Biological Sciences China Agricultural University Beijing 100094 China;

Department of Biological Engineering College of Life Sciences Delian Nationali;

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收录信息中国科学引文数据库(CSCD);中国科技论文与引文数据库(CSTPCD);
原文格式 PDF
正文语种 chi
中图分类植物学;
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入库时间 2024-01-27 03:06:43

Phosphorylation of Microtubule-associated Protein SB401 from Solanum berthaultii Regulates Its Effect on Microtubules

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