Effects of rutin on oxidative stress in mice with kainic acid-induced seizure

摘要

OBJECTIVE:Flavonoids are present in foods such as fruits and vegetables.Several studies have demonstrated a relationship between the consumption of flavonoid-rich foods and prevention of human disease,including neurodegenerative disorders.We assessed the effect of rutin(quercetin-3-O-rutinoside)on oxidative stress in kainic acid(KA)-induced seizure.METHODS:Thirty-six BALB/c mice were randomly divided into three groups.In the control group,saline(intra-peritoneal,i.p.)was administered for 7 d,and on the last day,KA(10 mg/kg,i.p.)was injected 30 min after administration of saline.In rutin groups,mice were pretreated with rutin(100 and 200 mg/kg,i.p.)for 7 d,and on the last day,KA(10 mg/kg,i.p.)was injected30 min after administration of rutin.Subsequently,behavioural changes were observed in mice.Lipid peroxidation and oxidative stress were measured respectively in the early and late phases after KA-induced seizures.RESULTS:Seizure scores in the rutin groups were significantly lower than those in the control group(P<0.01).Furthermore,rutin dose-dependently inhibited the number of wet-dog shakes(WDS)(P<0.05).Malondialdehyde level in the hippocampus of the rutin groups was significantly lower than that in the hippocampus of the control group on days 1 and 21 after KA administration.In the rutin groups,the thiol levels observed on day 1 after KA administration were higher than that in the control group(P<0.01).CONCLUSION:These results indicate that rutin has potential anticonvulsant and antioxidative activities against oxidative stress in KA-induced seizure in mice.